Gram staining

Method of staining bacteria developed by Hans Christian Joachim Gram in 1884. For this, the bacteria are stained with alkaline crystal violet for 1 - 3 minutes. After decantation of the staining solution, the bacteria are treated with iodine-potassium iodide producing a water-insoluble complex. Then, this solution is decanted as well. Afterwards, the bacteria are rinsed with alcohol. Results: Gram-positive bacteria retain the blue dye, Gram-negative release it. Gram did not carry out counterstaining with basic fuchsin or safranin as it later would become commonplace.

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The A-to-Z database provides information on each pathogen, the most common infections that it triggers, its main transmission paths and recommendations on disinfection. In the glossary, you will find explanations of infection control terms. Search now!

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