from Kampf G, Hollingsworth A 2003
published in J Hosp Infect 55(3):225-31
DOI 10.1016/s0195-6701(03)00298-6

Validity of the four European test strains of prEN 12054 for the determination of comprehensive bactericidal activity of an alcohol-based hand rub.

The four test bacteria of the suspension test for the EN 13727 (earlier prEN 12054) cover the entire spectrum of clinically relevant bacterial species.

The study evaluated the bactericidal activity of an alcohol-based hand rub (Sterillium) within 30 seconds according to prEN 12054 (now EN 13727) and in a time-kill test with 13 Gram-positive, 18 Gram-negative and 14 antibiotic-resistant bacterial pathogens. The EN 13727 tests were performed using ATCC strains of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterococcus hirae. For the time-kill test, 13 Gram-positive species, 18 Gram-negative species and 14 antibiotic-resistant bacterial pathogens were used to determine the efficacy of Sterillium. These included species such as methicillin-resistant S. aureus (MRSA), vancomycin-resistant enterococci (VRE), Staphylococcus pneumoniae, Acinetobacter baumannii and Klebsiella pneumoniae. In the EN 13272 tests, Sterillium reduced all four test bacteria by more than 5 log10 steps within 30 seconds. In the time kill test, all 13 types of Gram-positive bacteria, all 18 types of Gram-negative bacteria and all 14 types of emerging pathogens were killed by Sterillium by more than 5 log10 steps within 30 seconds.

Use disinfectants safely. Always read the label and product information before use.
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